Cell Biology

Stable vs transient expression: Which to use and when?

How Does Mycoplasma Contamination Affect Cell Culture?

Water-jacketed incubators: Do they really hold the temperature longer during power outages?

In this article, we look at the questions whether water-jacketed incubators offer advantages during power outages.

Water-jacketed incubators: Do they really provide a higher temperature uniformity for your cells?

In this article, we look at the questions whether or not water-jacketed incubators can really provide higher reproducibility of cell culture ...

Water-jacketed incubators: Can they provide a higher relative humidity?

In this article, we look at the question whether or not water-jacketed incubators can provide a higher relative humidity and the rationale b ...

Water-jacketed incubators: Disadvantages to consider

In this article of our series about water-jacketed incubators, we look at the disadvantages of this heat transfer technology.

Sample storage: Manage samples more efficiently

Well-organized biological sample management and storage is fundamental for any scientific study, but is especially imperative in clinical st ...

When You Think about Pipetting Liquids, Think Twice

Starting an experiment means asking many questions. Which material is needed? Which samples are used? Which conditions are necessary, e.g., ...

What is Primary Cell Culture?

How do cells cultured in 2D and 3D differ? A dimension shift

Cell seeding protocol – Guide on how to seed cells correctly

Cell seeding is usually the first protocol step and a standard procedure in cell-based experiments. A correct and standardized cell seeding ...

How to Remove Contamination in Cell Culture – Expand Your First Aid Kit

Contamination is one of the worst fears for scientists working in a cell culture laboratory. Biological contamination in cell culture is nea ...

How to thaw cells – Guide for more reproducible cryopreservation results

Cell thawing is an essential and sometimes undervalued element in all areas of cell-based research and production. A standardized cryopreser ...

384 = 96 x 4, isn’t it? - Save time with 384-well formats

It cannot be ignored: The workload in labs is constantly growing. 25 years ago, working with 96-well plates may have been progressive but no ...

Scaling Up Stem Cell Culture

In vitro, stem cells are traditionally grown on a 2D surface such as a T-flask or a cell culture plate. However, as this approach offers lim ...

UV-Vis Spectrophotometry – Easy and Quick Quantification of Nucleic Acids

The absorbance measurement of a nucleic acid solution at only four wavelengths is sufficient to determine its concentration and to obtain an ...

Verification of Photometric Measurement Values at a Glance

A quick check of the measured data based on the following key parameters increases the certainty that the results are reliable.

Tips & Tricks for Photometric Measurements

It is helpful to have in mind not only the photometric measuring process itself, but also its set-up, the measuring environment and the equi ...

Pipetting small volumes - How to tame the 384-well beast?

Do the tips fit? Will I manage to concentrate sufficiently on 384 tiny wells? Will I be able to fill the plate flawlessly? Do these concerns ...

Cell Culture FAQ: Sources of Mycoplasma - Where do they come from?

Have you ever wondered how mycoplasma get into your cell culture? Learn more about potential sources and helpful ressources in this article.

Cell Culture FAQ: How to handle various cell lines?

How ‘Pippi’ works as a team member in a protein analysis laboratory in Denmark - Automate your complex and variable protocols for protein analyses

Proteins are the most versatile and manifold macromolecules in nature. Our knowledge about proteins, their biological functions, therapeutic ...

How to prevent contamination in the cell culture lab

In order to prevent contaminations in cell cultures, it is imperative that equipment be thoroughly cleaned, or exchanged, after work with ea ...

How to identify eukaryotic contamination in your cell culture